Endophytic species of Colletotrichum associated with mango in northeastern Brazil

1、内容描述：关于内生炭疽菌的分类和鉴定方法的文献。和上一篇文章不同的是，本文的描述更为详细。对于使用不同的引物进行分类的方法讲解的更清楚。结论的分析也更具体。

2、适用人群：生物学专业的童鞋

3、内容摘要：Endophytic species of Colletotrichum associated with mango
in northeastern Brazil
Willie A. S. Vieira & Sami J. Michereff &
Marcos A. de Morais Jr & Kevin D. Hyde &
Marcos P. S. Câmara
Received: 17 January 2014 /Accepted: 7 June 2014
# Mushroom Research Foundation 2014
Abstract Endophytic species of Colletotrichum associated
with Mangifera indica (mango) are poorly understood. In this
study, Colletotrichum species were isolated from mango in
Pernambuco State, Brazil. There were significant differences
in isolation frequencies of Colletotrichum species among sites
and plant tissues. Mature leaf blades were colonized by most
Colletotrichum isolates at the majority of sites. Partial sequences
of glyceraldehyde-3-phosphate dehydrogenase
(GAPDH) of 97 Colletotrichum isolates were amplified as
an initial measure of genetic diversity. Phylogenetic analysis
with a subset of 22 isolates were performed based on a multilocus
dataset (ACT, TUB2, CAL, CHS-1, GAPDH, ITS)followed by Apn2/MAT IGS sequence-analysis for isolates
within the C. gloeosporioides species complex.

4、推荐理由：个人在完成毕业论文时用到的，认为还是挺有用的，分享给大家。我认为可以结合上一篇文献一起看。